Registration is open for the second RIDE24, an endurance relay for road racers at the Top Gear test track in Surrey.
Organisers are hoping to build on the success of this year's first edition and attract up to 60 teams of four riders.
The aim is to complete as many laps as possible of the floodlit airfield within 24 hours, and raise lots of money for children’s health charity Action Medical Research.
Event manager Sarah Stevenson, a keen cyclist, told BikeRadar that the Le Mans-style race was the only event of its type in the UK.
She said: "This year's event was a bit of a tester to see how popular it would be. We had 25 teams, lots of them from banks and blue-chip companies, and it was really hardcore.
"A lot of the guys who did it from Sky, Deutsche Bank and JP Morgan had done marathons and ironmans, and they said this was much, much tougher than that.
"There's something unique about riding through the night with your team. The camaraderie is amazing. All of the teams that took part this year have registered again."
The 2010 event will take place at Dunsfold aerodrome on June 19-20. Organisers are offering a 50 percent discount on the £240 team entry fee until the end of November.
Food and drink will be provided, and teams can take advantage of free massages, music and entertainment in the
It is hoped Olympic road race champion Nicole Cooke, cycling ambassador for Action Medical Research, will again make an appearance to present the prizes.
To get a taste of what the event's like, check out the video below: